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Biosera Ltd normal chicken serum
Normal Chicken Serum, supplied by Biosera Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/normal+chicken+serum/normal+chicken+serum/pm38879537-72-47-50
Average 90 stars, based on 1 article reviews
normal chicken serum - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Incubation:

Article Title: Experimentally induced testicular dysgenesis syndrome originates in the masculinization programming window
Article Snippet: .. Next, the sections were blocked in normal chicken serum (NCS; Biosera) diluted 1:5 in TBS containing 5% (w/v) BSA (NCS/TBS/BSA), followed by incubation with anti–SOX-9 antibody diluted 1:5,000 in NCS/TBS/BSA overnight at 4°C. .. The next day, sections were incubated with peroxidase-conjugated chicken anti-rabbit secondary antibody (Santa Cruz, catalog SC-2963), diluted 1:200 in NCS/TBS/BSA for 30 minutes at room temperature, followed by incubation with Tyr-Cy3 (Perkin Elmer-TSA-Plus Cyanine3 System; Perkin Elmer Life Sciences) according to the manufacturer’s instructions.

Article Title: Inhibition of WNT/β-catenin signalling during sex-specific gonadal differentiation is essential for normal human fetal testis development
Article Snippet: In brief, immunofluorescence was performed with TBS washes (3 × 5 min) between each step and all incubations were carried out in in a humidity box (Fisher Scientific, UK). .. Sections (4 μm) of formalin fixed tissue were dewaxed and rehydrated using standard procedures, followed by heat-induced antigen retrieval (pressure cooker) in 0.01 M citrate buffer (pH 6) and peroxidase block in 3% (v/v) H O in methanol for 30 min. Next, the sections were blocked in normal chicken serum (NCS; Biosera, Ringmer, UK) diluted 1:5 in TBS containing 5% (w/v) BSA (NCS/TBS/BSA), followed by incubation with COUPTF-II antibody diluted in NCS/TBS/BSA overnight at 4°C. .. The next day, sections were incubated with peroxidase-conjugated chicken anti-mouse secondary antibody (Santa Cruz), diluted 1:200 in NCS/TBS/BSA for 30 min at room temperature (RT), and followed by incubation with Tyr-Cy3 (Perkin Elmer-TSAPlus Cyanine3 System; Perkin Elmer Life Sciences, Boston, MA, USA) according to manufacturer’s instructions.

Article Title: Dynamic changes in DNA modification states during late gestation male germ line development in the rat
Article Snippet: Following re-hydration, tissues were submerged in 0.01 M citric acid (sigma, pH6) and pressure cooked for 5 min at 125°C. .. Endogenous peroxidase activity was then blocked by incubation in 3% ( v / v ) hydrogen peroxide/methanol for 30 min. Tissues were blocked with normal chicken serum (NChS, Biosera) diluted 1:5 with 5% ( w / v ) BSA in TBS (NChS/TBS/BSA), before incubation with anti-TDG (1:500, rabbit, Sigma) antibody overnight at 4°C. .. Following washing, tissues were incubated with chicken anti-rabbit peroxidase-conjugated secondary antibody (1:200, Santa Cruz, CA, USA) in NChS/TBS/BSA for 30 min.

Article Title: Inhibition of WNT/β-catenin signalling during sex-specific gonadal differentiation is essential for normal human fetal testis development.
Article Snippet: In brief, immunofluorescence was performed with TBS washes (3 × 5 min) between each step and all incubations were carried out in in a humidity box (Fisher Scientific, UK). .. Sections (4 μm) of formalin fixed tissue were dewaxed and rehydrated using standard procedures, followed by heat-induced antigen retrieval (pressure cooker) in 0.01 M citrate buffer (pH 6) and peroxidase block in 3% (v/v) H2O2 in methanol for 30 min. Next, the sections were blocked in normal chicken serum (NCS; Biosera, Ringmer, UK) diluted 1:5 in TBS containing 5% (w/v) BSA (NCS/TBS/ BSA), followed by incubation with COUPTF-II antibody diluted in NCS/TBS/BSA overnight at 4°C. .. The next day, sections were incubated with peroxidase-conjugated chicken anti-mouse secondary antibody (Santa Cruz), diluted 1:200 in NCS/TBS/BSA for 30 min at room temperature (RT), and followed by incubation with Tyr-Cy3 (Perkin Elmer-TSAPlus Cyanine3 System; Perkin Elmer Life Sciences, Boston, MA, USA) according to manufacturer’s instructions.

Article Title: Dibutyl phthalate induced testicular dysgenesis originates after seminiferous cord formation in rats
Article Snippet: .. The sections were blocked with normal chicken serum (NChS; Biosera) diluted 1:5 in TBS containing 5% BSA (NChS/TBS/BSA), followed by incubation with the first primary antibody, diluted in NChS/TBS/BSA, overnight at 4 °C. .. The next day, sections were incubated with the relevant peroxidase-conjugated secondary antibody, diluted in NChS/TBS/BSA for 30 min at room temperature (RT), followed by incubation with Tyramide (TSA-Plus Cyanine 3 System; Perkin Elmer Life Sciences) diluted 1:50 in its buffer, for 10 min. Before the next primary antibody dilution was added, the sections were subjected to antigen retrieval by boiling in a pressure cooker in 0.01 mol/L citrate buffer (pH 6.0) for 5 min.

Article Title: Nodal Signaling Regulates Germ Cell Development and Establishment of Seminiferous Cords in the Human Fetal Testis.
Article Snippet: Immunofluorescence Immunofluorescence was performed with Tris-buffered saline (TBS) washes (33 5 min) between each step and all incubations were carried out in in a humidity box (Fisher Scientific, UK). .. Sections (4 mm) were dewaxed and rehydrated using standard procedures, followed by heat-induced antigen retrieval (pressure cooker) in 0.01 M citrate buffer (pH 6) and peroxidase block in 3% (v/v) H2O2 in methanol for 30 min. Next, the sections were blocked in normal chicken serum (NCS; Biosera, Ringmer, UK) diluted 1:5 in TBS containing 5% (w/v) BSA (NCS/TBS/BSA), followed by incubation with OCT4 antibody diluted 1:100 in NCS/TBS/BSA overnight at 4 C. The next day, sections were incubated with peroxidase-conjugated chicken anti-mouse secondary antibody (Santa Cruz), diluted 1:200 in NCS/TBS/BSA for 30 min at room temperature (RT), and followed by incubation with Tyr-Cy3 (Perkin Elmer-TSAPlus Cyanine3 System; Perkin Elmer Life Sciences, Boston, MA, USA) according to manufacturer’s instructions. ..

Blocking Assay:

Article Title: Inhibition of WNT/β-catenin signalling during sex-specific gonadal differentiation is essential for normal human fetal testis development
Article Snippet: In brief, immunofluorescence was performed with TBS washes (3 × 5 min) between each step and all incubations were carried out in in a humidity box (Fisher Scientific, UK). .. Sections (4 μm) of formalin fixed tissue were dewaxed and rehydrated using standard procedures, followed by heat-induced antigen retrieval (pressure cooker) in 0.01 M citrate buffer (pH 6) and peroxidase block in 3% (v/v) H O in methanol for 30 min. Next, the sections were blocked in normal chicken serum (NCS; Biosera, Ringmer, UK) diluted 1:5 in TBS containing 5% (w/v) BSA (NCS/TBS/BSA), followed by incubation with COUPTF-II antibody diluted in NCS/TBS/BSA overnight at 4°C. .. The next day, sections were incubated with peroxidase-conjugated chicken anti-mouse secondary antibody (Santa Cruz), diluted 1:200 in NCS/TBS/BSA for 30 min at room temperature (RT), and followed by incubation with Tyr-Cy3 (Perkin Elmer-TSAPlus Cyanine3 System; Perkin Elmer Life Sciences, Boston, MA, USA) according to manufacturer’s instructions.

Article Title: Inhibition of WNT/β-catenin signalling during sex-specific gonadal differentiation is essential for normal human fetal testis development.
Article Snippet: In brief, immunofluorescence was performed with TBS washes (3 × 5 min) between each step and all incubations were carried out in in a humidity box (Fisher Scientific, UK). .. Sections (4 μm) of formalin fixed tissue were dewaxed and rehydrated using standard procedures, followed by heat-induced antigen retrieval (pressure cooker) in 0.01 M citrate buffer (pH 6) and peroxidase block in 3% (v/v) H2O2 in methanol for 30 min. Next, the sections were blocked in normal chicken serum (NCS; Biosera, Ringmer, UK) diluted 1:5 in TBS containing 5% (w/v) BSA (NCS/TBS/ BSA), followed by incubation with COUPTF-II antibody diluted in NCS/TBS/BSA overnight at 4°C. .. The next day, sections were incubated with peroxidase-conjugated chicken anti-mouse secondary antibody (Santa Cruz), diluted 1:200 in NCS/TBS/BSA for 30 min at room temperature (RT), and followed by incubation with Tyr-Cy3 (Perkin Elmer-TSAPlus Cyanine3 System; Perkin Elmer Life Sciences, Boston, MA, USA) according to manufacturer’s instructions.

Article Title: Human Adipose-derived Pericytes Display Steroidogenic Lineage Potential in Vitro and Influence Leydig Cell Regeneration in Vivo in Rats
Article Snippet: .. Binding of antibodies to non-specific epitopes was blocked by incubating sections with a blocking solution containing normal chicken serum (Biosera, Uckfield, UK) diluted 1:5 in TBS containing 5% (w/v) bovine serum albumin (BSA; Sigma-Aldrich Co. Ltd., UK). .. Sections were then incubated with primary antibody against the Leydig cell marker hydroxysteroid dehydrogenase 3-beta 1/6 (sc-30820, Lot #H0814; Santa Cruz Biotechnology, Germany) diluted 1:400 in blocking serum at 4 °C overnight.

Article Title: Nodal Signaling Regulates Germ Cell Development and Establishment of Seminiferous Cords in the Human Fetal Testis.
Article Snippet: Immunofluorescence Immunofluorescence was performed with Tris-buffered saline (TBS) washes (33 5 min) between each step and all incubations were carried out in in a humidity box (Fisher Scientific, UK). .. Sections (4 mm) were dewaxed and rehydrated using standard procedures, followed by heat-induced antigen retrieval (pressure cooker) in 0.01 M citrate buffer (pH 6) and peroxidase block in 3% (v/v) H2O2 in methanol for 30 min. Next, the sections were blocked in normal chicken serum (NCS; Biosera, Ringmer, UK) diluted 1:5 in TBS containing 5% (w/v) BSA (NCS/TBS/BSA), followed by incubation with OCT4 antibody diluted 1:100 in NCS/TBS/BSA overnight at 4 C. The next day, sections were incubated with peroxidase-conjugated chicken anti-mouse secondary antibody (Santa Cruz), diluted 1:200 in NCS/TBS/BSA for 30 min at room temperature (RT), and followed by incubation with Tyr-Cy3 (Perkin Elmer-TSAPlus Cyanine3 System; Perkin Elmer Life Sciences, Boston, MA, USA) according to manufacturer’s instructions. ..

Activity Assay:

Article Title: Dynamic changes in DNA modification states during late gestation male germ line development in the rat
Article Snippet: Following re-hydration, tissues were submerged in 0.01 M citric acid (sigma, pH6) and pressure cooked for 5 min at 125°C. .. Endogenous peroxidase activity was then blocked by incubation in 3% ( v / v ) hydrogen peroxide/methanol for 30 min. Tissues were blocked with normal chicken serum (NChS, Biosera) diluted 1:5 with 5% ( w / v ) BSA in TBS (NChS/TBS/BSA), before incubation with anti-TDG (1:500, rabbit, Sigma) antibody overnight at 4°C. .. Following washing, tissues were incubated with chicken anti-rabbit peroxidase-conjugated secondary antibody (1:200, Santa Cruz, CA, USA) in NChS/TBS/BSA for 30 min.

Binding Assay:

Article Title: Human Adipose-derived Pericytes Display Steroidogenic Lineage Potential in Vitro and Influence Leydig Cell Regeneration in Vivo in Rats
Article Snippet: .. Binding of antibodies to non-specific epitopes was blocked by incubating sections with a blocking solution containing normal chicken serum (Biosera, Uckfield, UK) diluted 1:5 in TBS containing 5% (w/v) bovine serum albumin (BSA; Sigma-Aldrich Co. Ltd., UK). .. Sections were then incubated with primary antibody against the Leydig cell marker hydroxysteroid dehydrogenase 3-beta 1/6 (sc-30820, Lot #H0814; Santa Cruz Biotechnology, Germany) diluted 1:400 in blocking serum at 4 °C overnight.



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